rubidium

You’ve taken a very small slice of the total workflow to develop cas9 crispr edits. Yes some components are “not crazy expensive”, but that’s not therapeutic production levels. And no sequencing. And no bioinformatics. And then zero tissue targeting/ delivery or safety studies.

So $5k is a massive stretch. I do think costs are coming down. But don’t want to oversell current state of “garage hacking” either.

I thought it supports most file storage sync solutions? I’m using with the free iCloud level storage I believe.

Yes. Aldevron and IDT are two companies (owned by danaher) that collaborated to make this happen. They have multiple authors listed in the NEJM article.

https://jobs.danaher.com/global/en/search-results?keywords=S...

More or less, yes.

An interesting part of the study was determining what a clinical dose _should_ be. You need enough to edit enough liver cells. But don’t really want to completely overdo it to limit potentially negative side effects. Seems like they got it right enough here, with the first dose having some effect and the subsequent dose having more.

It can make “bad edits” eg off target effects. But in this case there were, as far as is known, none. It’s aided that this was a single nucleotide defect.

They specifically tested for off target edits in the mouse study and found no harmful edits (and very rare off target ones). That plus the specific targeting of the liver cells (no germ line effect expected), makes this a low risk approach and certainly better than doing nothing.

Major diseases have been a part of human history throughout. There is no evidence that mega farming is making it worse.

Dude, the number of times outright lying has been done and brushed off in a 24 hour news cycle means it’s totally what they are doing.

Would you share what setup you use or recommend for this, both NAS and software for phone streaming?

You got books from the library to figure it out. Better than google slop these days?

Glad to see Ted Gioia’s work here. He writing over the last year has been helpful in getting new understanding for me.

His posts on technology are sane and levelheaded without being Luddite.

I have the paid version and it’s not connecting

Going way back to the start

Yes these were my questions too!

It’d be even better if it could be administered by a concerned offspring of an aging parent as they’re likely the first to wonder!

Um, no they’re trained to get in as fast as possible. Life >> cost of any door.

Historical homes are energy efficient. They don’t require any new resources to build, and will typically last much longer if they were built with old growth lumber.

Retro fitting is the way. Don’t tear them down for some new crap.

It depends on your role. If your just a SW dev on big team delivering some incremental feature its pretty dull. If you’re reporting to a director or above you can try fun stuff and push the envelope more.

This is one of the subjects that I upset about. How did it get so bad? How can we fix it?

I’ve tried DuckDuckGo but it fails due to spam too.

I think the only viable option in the next 5 years in a good LLM with a user (or community crowd-sourced) library on RAG. Ideally with privacy and local hosting options. I want to make “my library” (choosing which archives to trust as authoritative) and then put a good search interface over it. It’s totally possible now!

Any other takes on solutions? Anyone building what I describe?

UX started with hardware, it was just called human factors. And most of what they came up with then gets forgotten today.

Beg your pardon, but I think I would know (PhD in experimental AMO physics).

It was one of these: https://www.ipgphotonics.com/en/products/lasers/low-power-cw...

Agreed. A 40W 1550nm laser I used in grad school would burn post it notes readily and slowly burn black painted objects. It gave me a little burn once too (Was like touching a hot stove). 1.5W focused to 100 um would be a zinger, but at 4 mm would be not super dangerous thermally.

The main risk is you couldn’t see it so no blink reflex to help from even specular reflections.

Regardless, it would be reckless to expose this to people without eye protection.

My Maverick went up in price after I bought it at MRSP. But that was a market anomaly.

There’s usually a drop some but that amount is excessive.

QC is just as solid of theoretical footing at fusion. It is mostly a technical/engineering/materials problem with plenty of room for clever physics to make it easier.

“We therefore appoint the most possible mechanism to the superconducting vortices. The low-field absorption of microwave power with the assistance of dc magnetic field points to the small superconducting gap, and the relevant metastable excited states emerge to be vortices.”

It remains to be seen if this is a misunderstood experiment or a new discovery. Regardless, this is science operating as it should with people sharing the data that they have. But no need for the general public to speculate aimlessly.

No. That’s not actually how most orgs break it down. R&D, marketing, sales is “bringing new business” so are profit centers. This means their budget grows with revenue. Manufacturing, QA, IT and service are cost centers so get squeezed year-over-year even if revenue is flat.

I don’t get negative reviews to my knowledge :)

Maybe it’s the balance of regular coffee intake for lowering pH but a naturally higher pH?

Nothing that severe. I have beer, toast, sandwiches and burgers. I don't do a ton of sweets / soda but I do some (and if I do my teeth definitely get a fuzz that motivates brushing!).

My great grandma loved jello “salad”s (eg jello with fruit in it). It’s just genetics.

Anecdote time: My great grandmother lived to be 102 and never had a cavity. Kept all her teeth to the end.

One time, an orderly who thought she was just being senile tried to reach into her mouth to take out her teeth (thinking she had dentures). My great grandmother bit that person; hope they learned not all old people are senile!

I’m 40% of the way there. No cavities and rave reviews from my dentist. I brush—-best case —-once a day.

I’d not be surprised if it’s a genetic lottery of tooth enamel, tooth size (small) and mouth biome.

Some neighborhoods are pedestrian friendly but yes generally not. Biking ain’t great but downtown is making progress.

I’ll just add mine here:

Pros: decent chef-driven dining scene, active downtown due to sports and conferences, affordable, small enough to not feel like a major metro but big enough to have stuff to see/do, not a far drive to country areas to get out of town. As someone who grew up in a small town but then have lived in major metros since, I’m most comfortable in Indy.

Cons: distance to mountains (7 hr to Smokies) or oceans (13 hr to outer banks), relatively tame and limited local hiking options, potholes in spring, limited tech options (Eli Lilly, Salesforce, Rolls Royce, Cummins, Roche, Beckman, Corteva, Hospitals, … maybe a few others I'm forgetting).

Edina= “every day I need attention”, if I recall from growing up near there.

I disagree though. MN is colder but less windy so you get those crisp calm, blue sky winter days in Jan-Feb that make the winter more survivable. But you gotta be ok with the cold.

That all said, I’m now in central Indiana and prefer the milder climate (than both those places!).

I found it to be good for casual people economic history. Wasn’t overly “economics” in the technical sense.