harleyk

A recent report from the American College of Cardiology emphasizes the prediction power of hsCRP:

"High-sensitivity C-reactive protein (hsCRP) is an inexpensive and widely available blood test. While there has been debate within the medical community regarding the utility of hsCRP, this statement details the data confirming its value in clinical decision making in primary and secondary prevention."

https://www.acc.org/latest-in-cardiology/journal-scans/2025/...

Antibodies are proteins, so why is antibody design not just the same problem as mini-binder design? In most ways they are the same. The main difference is that the CDR loops that drive antibody binding are highly variable and do not benefit directly from evolutionary information the way other binding motifs do. Folding long CDR loops correctly is especially difficult.

Here I'll review the latest antibody design tools.

I'm forming a team to research AI and snake proteins for potential therapeutics. You can read more about our team's proposal on Discord.

https://discord.com/channels/1098625558780317766/12889513553...

The best writing I have ever done was in the acknowledgements section of my dissertation.

"...For all the experiments I’ve performed and the data I’ve collected, for none have I so unequivocally discovered truth as this: Love is eternal."

https://drum.lib.umd.edu/items/a0d83e35-0382-48b7-9183-c5aef...

It's hard to be a scientist and to be authentic in our current grant-funding race.

Being a dad is wonderful. Congratulations. My fatherhood (parent of four) has changed me in profound ways. The world glows brighter when we have a child with whom to share it.

I’m delighted that you’re searching for information about being the best dad possible. My advice, which has been supported by personal experience and research, is that the relationship with the mother matters. Marry the mother and stay married. More than any other variable, more than any other technique or quality of being a dad, a stable marriage (stable, enduring relationship) is the best quality we can give to our child. It is strong predictor of a child’s success and happiness. https://www.brookings.edu/articles/cohabiting-parents-differ...

This paper is a big deal. I appreciate the laborious efforts the authors underwent to calculate a more accurate infection fatality rate (IFR). The IFR is the number of deaths from a disease divided by the total number of cases. If 10 people die of the disease, and 500 actually have it, then the IFR is [10 / 500], or 2%.

The IFR is different for age-stratified groups. The IFR was calculated before COVID-19 vaccination became widely available.

It's a big deal because it reports a markedly lower COVID-19 IFR than previous studies. The panic may not have been warranted for non-elderly age groups. I read this to also mean that the risks to adolescents did not warrant school closures and for some ages vaccination, even though vaccination risks for this age group were minimal.

"...in many locations excessive hospitalizations may have been driven by irrationally high perceptions of IFR for non-elderly people and they may have caused unnecessary stress and damage to the health care system at large."

Aileen Neilsen did a 3 hr session discussing timeseries analysis SciPy 2016. Each chapter is a Python Notebook. The ARIMA model figures prominently in chapter 7 Forecasting. I find her session tremendously helpful when analyzing ts data especially her discussion of seasonality. Youtube walkthrough: https://www.youtube.com/watch?v=JNfxr4BQrLk Github data: https://github.com/AileenNielsen/TimeSeriesAnalysisWithPytho...

Really happy for Solugen's success and what this means for other biotechs seeking to augment traditional petromethods with enzymatic ones.

Can you help us better understand how you use CRISPR/Cas9 to mutate the protein? Your enzyme is proprietary, sounds like human protein, but mutated in certain positions prob to increase rate, but how do you use Cas9? I assume like other biotechs you purify this single protein using either E. coli or yeast with protein on plasmid. So why use Cas9 instead of site-directed mutagenesis?