HNHacker News
TopNewBestAskShowJobs

aFLH7U2daj

25 karma · joined January 24, 2018

submissionscomments
aFLH7U2daj··on Nanopore MinION – $1k solid-state DNA sequencers
Flow cells are 1000$ each (unless you buy a lot of them), the sequencer is basically a USB dongle with a uC in it and all the important stuff is in the flow cell. Flow cells are basically disposable (but you mail them back). You get one-ish use out of a flow cell though they can be "washed" and realoaded.

Reagent kits run a few hundred dollars depending on what your doing and you get several uses out of them.

IIRC it's not certified for use as a medical diagnostics device so it's "research only".

It's a nice system with great (none) upfront costs that has a lot of potential. But read quality and quanity per dollar aren't as good as what we get with our NextSeq (which by contrast costs something crazy like 300-400k)

aFLH7U2daj··on Nanopore MinION – $1k solid-state DNA sequencers
You're buying a service for the 500-800$. Here you're buying a flowcell for 1000$ (the "sequencer" is more or less free). The technology is pretty different and a lot more bleeding edge. If what you want is just a genome aligned to a known reference and you can wait a few days then this is not what you want.

We use ONT's minION for strange niche stuff, where either the DNA prep method matters or the latency (the time from loading to the first data out of the machine) matters. Read quality is typically not as good as illumina, so for applications where we need quality we stick with the NextSeq. There's no reason to think nanopore technology won't get better though, but for now, if you've got the money illumina is still the way to go most of the time.