For example, it would behoove a large company to spend a great deal of time optimizing say, their JSON parsing library. Although it may not identify as a hotspot in any one place in their immense codebase, it's extreme prevalence causes performance degradation subtly but pervasively.
I also measured injected object creation using Guice to be 40x slower than a simple constructor in java (agree or disagree with the 40x, but using reflection to set a variable instead of simple object construction is intuitively far slower).
Guice may not show up on any profiler as problem - but if you slow down object creation by a factor of 40x, something you may do thousands of times per second for the life of your program, you are degrading performance across the board. Rather the same if you simply clock your CPU down a few hundred Mhz.