That's why "open with a joke" is so useful. It's not really for them! It's for you!. If you can hear a laugh from your audience, YOU start to chill out. Your body is like "ah. the mic is actually on, and I'm not having a stroke".
Another useful tip, if the scenario allows, try to hang out by the front row before your talk and chat someone up. That's who you want to then make eye contact with often during your talk. Their expression will be or at least seem to be warmer to you. And you'll feel some more calm looking at them than the random heads everywhere else.