Then you have to classify them as 0's or 1's. Each is visually distinct, a 1 being encoded by the presence of a transistor and a gap in the polysilicon. We didn't have to guess which is which is by the nature of Intel microcode we could assume 0's were much more frequent, so a transistor meant a 1.
There are some automatic tools designed to perform this work via color thresholding, but they didn't work very well here because some of the mosaic was blurry, and a lot of dust had crept in which created false 1 bits.
Instead, we trained a convolutional neural network to classify the extracted bit regions into 0's and 1's. This was overlaid back onto the original mosaic as white or black squares at 50% opacity.
Then we spent several long, tedious days just checking the results for errors. Finally we had the raw 2d array of bits - the next step is to extract the microcode words from the bit array.