It likely depends how you're defining separated. Some cities go for completely separate routes, some curb separation, some bollard separation, some on-footpath separation. Some use a chaotic mix (Dublin, say, is building a 300km segregated system, but because this is being delivered by seven local authorities plus the National Transport Authority, it is a mix of seemingly every possible solution, including weird stuff like contraflow bike lanes, and bike lanes between on-street parking and the footpath).

Berlin certainly qualifies. If not by metric, then by vibe. Lightyears ahead of Chicago which I rate as a good city to bike in the US (and getting better under the current batch of aldercreatures).

143km sounds like quite a bit though, especially since separated bike lanes are usually for main thoroughfares, whereas many low-traffic side streets you simply bike down the middle.

Malmö has some of the best bike lanes in the world, IMO even better than Amsterdam and Copenhagen, who I'd say are top tier. Literally 2 meters wide at some points and separated by divider from even walking traffic