1. Your order can legally be “front run” by the lead or designated market maker who receives priority trade matching, bypassing the normal FIFO queue. Not all exchanges do this.
2. Market impact. Other participants will cancel their order, or increase their order size, based on your new order. And yes, the algos do care about your little 1 lot order.
Also if you improve the price (“fill the gap”), your single 1 qty order can cause 100 other people to follow you. This does not happen in paper trading.
Source: HFT quant