> samples from surviving mice on day 12 were also obtained through necropsy to measure viral titers.
"Recombinant viruses were generated using a pHW2000 plasmid-based reverse genetics system."
"Combining the R90K and H110Y mutations (22W_KY) resulted in a synergistic increase in thermal stability and maintained HA activity without measurable reduction even after 4 h at 52 °C."
"22 W HA and 22 W NA genes, along with six internal genomic segments (PB2, PB1, PA, NP, M, NS) from PR8 and a PB2 gene from 01310 containing the I66M, I109V, and I133V (MVV) mutations"
The study also confirmed enhanced antigen uptake and intracellular penetration in human cells:
"The highest level of intracellular entry was observed for BEI_22W_KY, confirming its superior effectiveness in penetrating cells."
Ref: https://virologyj.biomedcentral.com/articles/10.1186/s12985-...