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Another T1D here. I do not have a compatible pump with looping. But I'd like to dip my toe into openaps.

I use a cgm (libre2).

Can I use autotune to tune my carb ratio, basals etc. without looping? How was your experience in this?

Do I have to use nightscout to run autotune?

I've run it against my nightscout data a few times to get insights to my profile. So yes, you should install it somewhere and run a CGM app such as xdrip which can transfer your libre2 data to your nightscout database.

So yes, if you are interested on autotune, a nightscout is required for now.

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