There have always been multiple cultures. This is such a weird statement I don't even really know how to argue against it since to me it seems so self evident, but I suppose I'll try. Since the subject is music, I suppose we can start there.
The sort of people you found at, say, a metal show in the 80s and the sort of people you found at a dance club playing the early era of house music were wildly different. Even if you keep it within rock, the overlap of the audience seeing The Cure would be wildly different from those seeing Judas Priest.
Anime? Even before the internet became what it is today, you could go to a comic book store and hang out. There was significant overlap between the comic book/dungeons & dragons/anime crowds.
There have been social spaces and places to meet people within specific cultures for forever.