Yes I've tried it (pre Play 2), and there's one thing I don't like - favoring/integrating certain tools in it. I know that it uses Hibernate through the JPA API, and if you want to use a different ORM you'll have to make a lot of workarounds. And it's not only about ORM. A month or so ago I read that it will integrate a certain JavaScript library in it too. Tapestry Web Framework had this attitude, but the developers found that it brought more negative than positive effect, I hope Play will see the same. Modularity and ease of configuration is a very important thing.
I like it's Scala support and enthusiasm though, and I will definitely try it some day again.