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Comment by csdvrx | Hacker News Reader

It says in the first line "The integrated stress response (ISR) is activated during aging"

Odds are the same basic mechanism is activated by many other things, including some inflammation process.

Yeah, I am considering purchasing ISRB "for research". Some people I love suffer with chronic inflammation due to various autoimmune diseases. I know plenty of amateur body builders buy chemicals "for research" but use them as supplements. They keep tabular and video artifacts like most body builders do, so I wonder to what degree the activities are illegal?

The problem with ISRIB is that it isn't water soluble. I'm not convinced that people who take it (usually subcutaneous injection or snorting it) aren't just experiencing placebo. I don't know of any way of actually getting the molecule to your body's cells in a consistent manner.

The study dissolved it in DMSO, then that in polysorbate 80, then that in polyethylene glycol. Then injected that intraperitoneally. That seems doable.

Intraperitoneal injection isn't really done in humans, the closest equivalent would be subcutaneous or maybe intramuscular injection. I don't know about the safety of injecting DMSO into humans but it's probably not too bad since it seems perfectly safe when used for transdermal drug delivery. The bigger problem is that you're injecting a water-insoluble compound mixed in a small amount of solvent into what is essentially a big bag of water. Once that DMSO/PEG/whatever is injected, it will just mix with the water in your body, so the ISRIB will just precipitate out of solution since it is now surrounded by 99% water molecules. So it will never get distributed to all your organs, which is the goal.

I could be missing something, but since your cells are separated by water, it doesn't matter how many steps you take to get it in your body.

DMSO is soluble in both polar and non polar solvents. I assume the insolubility in water is precisely what they used it to solve. Im any case, their delivery mechanism worked in mice, so it would work in people. The part that might not carry over to people is the effect once in the cell.

I am cringing so hard imaging someone injecting something intraperitoneally. Good luck missing your intestines.

No idea, but unless it's on a restricted list (classified by the DEA), I think "not at all".

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