> The results show that the number of insects sampled on vehicle number plates in the UK decreased by 58.5% between 2004 and 2021, and that these differences were statistically significant. A comparison of the 2004 national data with the 2019 data from Kent showed a 53.7% decrease. The greatest decreases in splat rate between 2004 and 2021 occurred in England (65%) whilst journeys in Scotland recorded a comparably smaller decrease (27.9%), with intermediate decreases in Wales (55%).