When using a tuner to tune a guitar, you get the equal temperament normally desired for it's ability to play equally in any key. When tuning by ear, if you are just minimizing the beating you hear (instead of targeting the beating that exists in equal temperament, like good piano technicians are trained to do) you'll end up with integer ratios. Accidentally ending up at the integer ratio of a major third is "farther off" than doing so for a perfect fourth. Typically this manifests as a difficult time tuning the B string though, not the G string, if you go low to high. Solution: tune low to high for the E,A,D,G and tune high to low for the high E,B (doing a perfect octave for the E to E where the equal temperament ratio [4] perfectly matches the integer ratio [5]).
[0] 1.334840:1 or 2^(5/12):1
[1] 1.333333:1 or 4:3
[2] 1.259921:1 or 2^(4/12):1
[3] 1.25:1 or 5:4
[4] 2:1 or 2^(12/12):1
[5] 2:1