When using a tuner to tune a guitar, you get the equal temperament normally desired for it's ability to play equally in any key. When tuning by ear, if you are just minimizing the beating you hear (instead of targeting the beating that exists in equal temperament, like good piano technicians are trained to do) you'll end up with integer ratios. Accidentally ending up at the integer ratio of a major third is "farther off" than doing so for a perfect fourth. Typically this manifests as a difficult time tuning the B string though, not the G string, if you go low to high. Solution: tune low to high for the E,A,D,G and tune high to low for the high E,B (doing a perfect octave for the E to E where the equal temperament ratio [4] perfectly matches the integer ratio [5]).
[0] 1.334840:1 or 2^(5/12):1
[1] 1.333333:1 or 4:3
[2] 1.259921:1 or 2^(4/12):1
[3] 1.25:1 or 5:4
[4] 2:1 or 2^(12/12):1
[5] 2:1
I assume you are talking about fretless instruments. With a guitar you just play a unison using the frets and the thicker string.
Rather, I'm talking from a point of view of playing two adjacent open strings. Or harmonics thereof (as some people like to tune with 7th fret harmonics and so forth). If you aren't fretting and you minimize beating down to nothing, then you end up with integer ratios.
I think you're right about the "beating"... in general this post has been extremely instructive for me. My teachers were piano players. Either they didn't know better (because they didn't need it) or they didn't bother to explain the fine details to me. Even the books I consulted later don't tell the whole story. That's the magic of the Internet :-)
In contrast to matching the same note on the next string?
But recently I discovered the most helpful trick, use a fifth from G down to the A string, and also use a fifth from the D to the low E string. Those last two intervals can be the most surprisingly out of tune intervals when using either ear tuning or an electronic tuner.
When you play B and E open and tune them to eliminate beating, you're in okay shape because you're making a "just" (integer ratio) perfect 4th which is not terribly far from an equal temperament perfect 4th.
See my earlier comment [0] with all the numbers if you want to quantity "pretty bad" vs "okay". In that comment, my initial references to footnotes 3 and 4 should actually be 2 and 3, but it's too late to correct it.