I'm going to sidestep the rail & bicycle hole here and focus on busses since those are the closest alternative in America specifically. I wish bikes were more feasible nationwide though.
There are plenty of population centers that aren't densely urban. Cities in the 200k to 400k range seem like they're just starting to be big enough to support a regional bus system on their own, or with the support of a nearby larger city.
The problem is how infrequently they would run though. Those cities tend to sprawl just a bit as they haven't made the full transition to multi-tenant dwellings so lots of folks would have to walk multiple blocks sometimes to get to a stop.
Individually operated vehicles, namely cars, are still the name of the game for the vast majority of America. But then you run into the electrification issues I mentioned. A lot of these neighborhoods have very old electrical systems, and while we've made a ton of room by not running 60W light bulbs everywhere, I'm not sure we could handle charging 50k electric cars per night in many medium sized cities.