https://journals.plos.org/plosone/article?id=10.1371/journal...
Heres a decent open source? stage that i adapted for one of my projects in the past. Resolution is 1 micron laterally. If i remember correctly they address several issues including stepper motor backlash and micro-stepping. Cost can be well below the $1000 they quote depending on the parts you choose, or e.g. if you do only two axis (xy) stage instead of three (xyz)