Not really, in many places we actually still have the (disused and unmaintained) rail for last mile industrial delivery, which used to be the norm. Maybe at one point it would have required land use expansion, but we already paid that cost in the previous century.
Overall rail is generally going to be much, much more land efficient than roads. Yes, rail has a limited turning radius that must be accounted for[0], but rail can also deliver a lot more cargo than a road can for less maintenance. The Union Pacific indicates that the maximum weight for a shipment is 268,000 lbs per car. Meanwhile a semi-truck cannot exceed 80,000 lbs pretty much anywhere. A single freight train can pretty easily deliver a couple thousand tons of cargo, something that would take a hundred semi-trucks to deliver. This will almost certainly result in extra lanes and parking facilities for semi-trucks, increasing land use.
And then there's the fact that semi-trucks are really, really hard on roads. They do the overwhelming majority of the damage to them, due to their weight. From an infrastructure cost perspective, the best thing you could do is try and shift as much industrial shipments off of roads and onto railways. Obviously last mile will always be an issue, chances are your local target will be stocked via truck, but we could at least connect factories and ports via rail.
0 - Then again, so do highways.