While running, yes. On Windows if you enable Hyper-V support, even when you're not using it at the moment, you can't use VirtualBox without reboots and shit.
Wait really? That doesn't seem right, why would they have the host running under part of hyper v when hyper v is optional? This would mean the entire foundation shifts when you enable or disable hyper v
To say nothing of the madness of using it on a laptop with several network adapters when the guest OS only wants to acknowledge one.