* Network ACLs, which describe the ruleset (consider it like a stateless firewall) for subnets and their respective routes. Whilst they are optional, having a default set it straightens out a lot of duplication that may end up in Security Groups (which are more stateful in nature).
* Elastic (public) IPs. NAT instances/gateways require their use, and there is dance to be done around their allocation in account, and attaching to instance interfaces.
* IPv6 components. Egress-only Internet Gateways operate differently to IGWs, as there is no NAT they need a route applied across all subnets both public and private. IPv6 CIDR which allocates the VPCs /56 (and thus each subnet gets a /64, and each instance's interface thus gets a /128 which is bananas, but IPv6 is a second class citizen on AWS). Finally updating the subnets so automatic IPv6 address assignment happens.
* VPC Gateways - these are broken into two types, the older type that support S3/DynamoDB and effectively allow traffic in a public/private subnet to bypass NAT. These enabled can have significant advantages to access and throughput. The newer "PrivateLink" services are different and having pricing costs associated with them.
* DNS and DHCP: It's a rule in the VPC that the delegated resolver lives on ".2" of the VPC's CIDR, and operates in dual-horizon - EC2 hostnames setup accordingly resolved by instances inside the VPC will get the private VPC CIDR address, not any Elastic IP.