Definitely. My company requires the use of standard Windows applications - no apps on mobile devices - that have fairly intuitive interfaces for me (a late 30's former software developer), and while they have quirks, I get around them decently enough.
Many of my younger employees have never owned a computer. Literally. They use mobile for everything and only used the computer labs to do word processing and the like in college, or had work computers. And they are incredibly inefficient and basically useless when they encounter these applications for the first time; it is a huge training cost that I didn't expect going into it.
But it makes sense, of course. My industry doesn't make applications for today's end users, it makes them for the people that suffered through the DOS/Windows 3.1/Novell Networking era, because most of their consumers are 30-50 years old in academia or large business.
This split is happening in more than just my industry; it's happening in a lot of others due to the fast pace of mobile adoption and abandonment of the traditional PC. The real fear is that my employees are no better at using mobile devices than I am; in theory they should be, but in reality they're just technologically far worse than anyone else who had to grow up using complex systems. I have long-term fears about what this will do to the population; many people have long assumed that generations continue to get smarter and more tech-savvy, but I have found this to be very, very false in my limited experience.