Best thing I ever did for tmux was change the command key prefix to "C-\". With caps lock mapped to control, this makes tmux commands available with almost no hand motion, just a slight reaching out with the pinkies. It also restores access to C-b for "back one character" in emacs and all programs using emacs key bindings, which is hardwired into my fingers at this point. (You're blocking emacs' function "toggle-input-method" - "Enable or disable multilingual text input method for the current buffer". For some users that would probably be an issue, but hasn't been for me.)
Another useful thing is to "bind-key C-\ last-window". That makes C-\ C-\ jump back to the last window you visited, which facilitates creating short-lived windows for specific tasks.
Final small tweak I like is "set -g base-index 1" so that C-\ 1 is the first window, 2 is the next, etc. instead of having the first window way over on 0.
My other tweaks are probably more idiosyncratic to my workflow.