Splitting hairs, but there's an assumption there that you're trying to react to the audience, often intending to please them. Coming from a background of DJ's playing "difficult" music, sometimes the audience's reaction is incidental.
I reckon the trick is in pacing the set and planning your first two or three records to keep most of them on board. If people have paid to get into the venue they must be at least a little predisposed to like what you're playing, and ideally you should be able to keep people dancing against their better nature.
Of course clearing the floor and then drawing them back in is always good too.
If you really don't care what they think then IMO it's perhaps more into the realm of performance than DJing. It's a tricky one.