Size of Ethernet frame - 24 Bytes
Size of IPv4 Header (without any options) - 20 bytes
Size of TCP Header (without any options) - 20 Bytes
So total size of empty TCP datagram - 24 + 20 + 20 = 64 bytes
total size of TCP datagram with 1 char - 24 + 20 + 20 + 1 = 65 bytes