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Is there an accuracy problem in PacBio's published de-novo assembly human genome: http://blog.pacificbiosciences.com/2014/02/data-release-54x-...

it was assembled de-novo (IE, without HuRef), and the accuracy stats aren't horrible.

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PacBio is ultimately more accurate than Illumina given consensus. Illumina has some systemic bias so the ultimate accuracy tops out around Q40-Q50 (same errors just repeat themselves) and PacBio will get you closer to Q60-Q70 because the random errors resolve out. So it's the most accurate platform actually. This singuler MinIon read however was barely mappable to the reference, so it looks pretty poor quality. But without more reads we don't quite yet know what the systemic error profile will be though for it. That's why they're adding more pore types, they say, to get multiple systemic error profiles to try to cancel some out.

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