Disabling root login without changing to using ssh-keys for authentication doesn't really buy you a whole lot of security; you can still access the box and root with knowing just one password -- the only thing you've avoided are brute force attempts against the root account.
With sudo and only keys based access, the attacker will need to get a copy of the key (barring any new problems with key generation, like the Debian ssl bug) and the password needed for sudo access (or the root password, for su -).
As other's have said, use visudo (possibly with "EDITOR=nano visudo) -- to avoid leaving your sudoers file in a broken state.
An as others mentioned -- make sure you can login and sudo before you log out of the root shell...