Largely, no, because it would be a spectacular waste of their time. Why? Because the regulations are usually microscopically focused on class of actors that (a) know they exist, and (b) have a vested interest in staying current.
Moxie Marlinspike's example of the undersized lobster rule is especially telling in how it goes against the very point he was attempting to make. On the enforcement side, this particular regulation likely affects fewer than 10,000 people in the entire country -- that is, people fishing commercially for lobsters. The public interest it serves is that fisheries are subject to the tragedy of the commons[1], and must be regulated to preserve these public goods. It's basically the equivalent of that little tag on your mattress that says "ILLEGAL TO RESELL" -- people joke about getting arrested for this. No, it's targeted at the vanishingly small number of people that sell mattresses commercially.
[1] http://en.wikipedia.org/wiki/Tragedy_of_the_commons
EDIT: Also, just a note -- laws are passed by Congress. Regulations are promulgated by agencies that have been delegated some task or authority from Congress ("we don't have time to draft/debate/vote on every little piece of trivia regarding airplane part advisories or what plants are on the invasive species list or what next year's technical specifications for automobile safety are -- FAA, USDA, NTSB, you guys have the experts and scientists, so here's your authority, now go and figure out the details and make it happen")