Tracking pixels don’t even work with Gmail because Google fetches them out of band. It doesn’t reveal open rates.
They also send you an email back saying your email wasn't delivered after a few days or hours, so there's little benefit in using the tracking pixel to determine if an address exists.
Don't know if they fetch images from emails sent to non-existent addresses, but I would if I were to design such a system.
"Our open rates have skyrocketed! send more emails!"
$ head -n 100 /dev/random | md5sum
a6cc1b7c09ccb122cb066c89e16b3140 -
And that yields an instantaneous error message https://i.imgur.com/twHhIU3.png that reads "Address not found. Your message to a6cc1b7c09ccb122cb066c89e16b3140@gmail.com was not delivered because the address could not be found".