Wasn't there recently a discussion of a risk of methanol formation from an ethanol producing strain? I sure hope that none of your strains produce ethanol.
We evolved LPS detection so long ago that it's in our innate immune system instead of adaptive immunity. It's so ancient we share this immune function with fruit flies.
LPS detection is so good and immediate because it's tuned to pick up single instances of LPS molecules. Not a few nmol. Single molecules. Detection will trigger inflammation and immune scale up to deal with the problem.
If you go injecting LPS or E coli into your blood stream, of course your own body is going to kill you. It'll freak out and think WW III has started and begin firing the nukes in every direction to stop it.
This is septic shock.
Methanol is produced by a lot of bacteria, almost every human produces it within their body.
Chemically the problem with ethanol is that it's too close to methanol.
”Small amounts of methanol are present in normal, healthy human individuals. One study found a mean of 4.5 ppm in the exhaled breath of test subjects.[19] The mean endogenous methanol in humans of 0.45 g/d may be metabolized from pectin found in fruit; one kilogram of apple produces up to 1.4 g of pectin (0.6 g of methanol.)[20]”
”Ingestion of as little as 3.16 grams of methanol can cause irreversible optic nerve damage, and the oral LD50 for humans is estimated to be 56.2 grams.[66]”
19: https://iopscience.iop.org/article/10.1088/0967-3334/27/7/00...
20: https://onlinelibrary.wiley.com/doi/10.1111/j.1530-0277.1997...
66: https://aoemj.org/journal/view.php?doi=10.1186/s40557-017-01...