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Do you happen to know if this change can affect people who have disabled systemd's Predictable* Network Interface Names before upgrading to Trixie?

*haha

You can easily keep the current naming behavior with the 'net.naming_scheme=' kargs

I already have systems that use net.ifnames=0. My question is about whether this new behavior can affect them.

It'll not, the new behavior is just a new naming scheme, and you just choose not to use any, which is totally fine if you have a single NIC.