Assume the person hasn't even read your homepage or even your emails. If there's multiple people in the call then at least one has no idea what the call is about. All busy people. You might have to explain everything again so have the basic slides ready. I had calls with existing customers (paying for years) where we had to re-explain who we are.
Enterprise has many departments. Figure out who the decision maker is, though likely the CFO is in your case, and who he needs to ask for input. There might be a security review you have to pass, your company might need to be added to a vendor database (questionnaires, tax ids), legal review.
Make up your mind on payment terms. They probably want to pay by invoice (bank transfer), not credit card. 30 days, 60 days? Custom termination period? Do they have a standard supplier contract you can review?
The question of company age, stability and number of employees might come up. Have answers prepared. I'd stay with the truth though, maybe claim you have contractors on stand-by. Once we had to submit all our code to a code escrow service (they burned our github repository to a CD-ROM) "in case of bankruptcy". Made sense, they paid for it.
Good luck!