Close - this is a SDS-PAGE gel, and you run it using proteins. The bands in the first two rows are from a western blot (gel is transferred to a membrane), where you use antibodies against those specific proteins to detect them. The Pon S row is Ponceau S, a dye that non-specifically detects all proteins - so it's used as a loading control, to make sure that the same amount of total protein is loaded in each lane of the gel.