Yeah, "you guys" is a fascinating case. I happen to be non-binary and autistic, so it's one that causes me a minor mental stutter to hear.

I absolutely never assume ill intent. But it does cause a cd-skip mentality for me, so I usually privately tell people. "Hey, heads up, you guys causes me an unfortunately unavoidable mental hiccup. It's not major, and I recognize it's common language. If it's low cost to you to use an alternative, awesome, but I don't want to cause an even bigger hiccup for you."

My goal is always to share the impact, and to be open to solutions that might include "nothing changes". I assume people would want to know they are unintentionally having that impact and also that I'm not going to roast them for using a very very common expression.