Most guitars are not properly in equal temperament intonation, because that requires compensation at the nut.
In particular, an unwound G string can be off. I got sick of three decades of listening to that out of my main guitar, and earlier this year finally did something out of it with satisfactory results:
https://mstdn.ca/@Kazinator/112332540341043265
Without this string, I cannot get these G and D power chords to be in tune simultaneously:
G:
E |---|---|-*-|
B |---|---|-*-|
G o|---|---|---|
D o|---|---|---|
D:
E x|---|---|---|
B |---|---|-*-|
G |---|-*-|---|
D o|---|---|---|
If you tune the latter D, then the open G is too flat in the former G. If you sharpen the open G, the latter D is out of tune.
The problem is that when we fret the A note on the G string near the nut, it stretches; the string goes sharp. So the interval between the nut and that fret is wider than a tone, without the compensation that shrinks it.
The other strings have the problem, but the unwound G has it worse due to lower tension relative to mass. I'm so happy with the G compensation on that guitar, that it needs nothing else.
Anyways, there are videos out there which do A/B comparisons between ordinary guitars and just intonation. They feature uncompensated guitars, so they perpetrate a fallacy.
The guitarist must experience a properly intonated guitar with nut compensation first, then decide whether going to exotic intonations is worth it.