According to the article “you” was formal and “thou” informal.
single plural used in contexts of
thou ye nominative (subject), vocative (preceded by the word "O"). For the singular, this implies that the relevant verb takes the "-[e]st" suffix.
thee you all object forms (direct object, indirect object, object of preposition)
thy your possessive determiner, before consonant sounds - thy will, thy God
thine your possessive determiner, before vowel sounds (including 'h') - thine enemy, thine head
thine yours possessive pronoun - not my will but thine, all that I have is thine
(the last distinction used to by used for my/mine, and is still used for a/an)