Mineplex had the most concurrent players but they weren't on a single individual bare metal server or single server jar instance.
A lot of large "servers" like Mineplex, Hypixel, etc run a proxy which sits in front of a bunch of other servers. The concept of a "server" can have many meanings in Minecraft so it gets fuzzy quickly.
I'm not sure if this 1,000 person test is a record but I'm not aware of any other test running so many concurrent players on a single dedicated box with a single instance of Minecraft running. Folia takes advantage of more CPU threads so everyone is in sitting in the same server instance.
Hypixel beat out Mineplex later on, BTW.