My opinion on this is:-
1. Forget the subs. Let people play for fun for free and if they want to bet take a cut of the ante or pot or something (IE you only make money when people spend money and even then only make a little). Make it very, very easy to bet (Paypal, google checkout easy). But let people play for Kudos too.
2. Also let people play on Facebook. Do Facebook Connect (or other social networks) so peeps joining your site can bring all their friends (their whole social graph). Make it so people can play away from your site basically (iPhone etc)
3. Bring in non-players IE have tutorials, novice tables, private game rooms stuff like that. There is a huge untapped market of women who love to gamble (witness the power of Bingo in the UK) if it is fun and social.
Personally I'd back you whatever as your drive and commitment are evident. Whether you follow my advice or not I expect you to succeed with this or another idea and if I had a few 10s of thousands of pounds I'd be in like Flynn. Sorry if that embarrasses you.