No, not really. I specifically ruled out .NET because BizSpark expires after 3 years or >1 million USD in annual revenue (whichever comes first).
I just don't see a ton of advantage to running .NET/SQLServer/etc over something like Java/PostgreSQL/etc. I'm sure some .NET dev will disagree.
There's just so much flexibility in being able to spin up as many instances as I want without licensing restrictions. For example, a .NET deployment might require larger instances to decrease the per-machine licensing costs, whereas I can do whatever-the-hell-I-want with an open source stack.
BizSpark is free only if you expect to not be around in 3 years.