The big different is the language and I think Nix wins here by a mile. Doing everything with Scheme just leads to layers of macro spaghetti that I really did not enjoy to dig through. Error messages that tell you absolutely nothing about what went wrong were pretty common. Nix has those too, but less frequently. Also with Nix you just use regular shell scripts snippets for the building the packages, Guix wants you to do it all in Scheme. Package selection on NixOS is much bigger.
Another big thing, Nix has Flakes, which make it trivially to turn all your Git repositories into Nix packages. Your Git repository becomes essentially a first class citizen in the package manager, making it completely trivial to run different versions of the same software. Guix has none of that, they still treat packages as a separate thing from the software itself and trying to add third party packages involves quite a bit of overhead. Easily up or downgrading individual software isn't possible as far as I can tell, you have have to roll back the complete Guix system to do so.
Basically, after switching from Guix to NixOS, I can't say I missed anything. NixOS just felt like a more polished and feature rich version of what Guix was doing, which given that Guix is basically a NIH version of Nix, is understandable.