Colony 8 was the first to die, which was not surprising, considering 8 was low aggression and their closest neighbor was Colony 1 which was high aggression, medium freedom and low energy. Shortly after, Colony 1 became the first colony to reach the cap of 500 ants. This was no doubt due to no longer having much conflict, and also obtaining their closest neighbor's resources.
The next to go was 6, which was an all-medium build pitted against 7, who I suspected at the start would be the winner, with max aggression, medium energy, and the most focus (lowest freedom). 6 put up a good fight, having killed just under 800 ants before succumbing to 7.
Colony 3, the other all-medium build, was the next colony to reach the population cap. I thought this juxtaposition was interesting, although not terribly surprising since their closest neighbor was 2, a low aggression and high-freedom build.
Colony 5, a medium-aggression build, struggled to stay afloat, which I thought was surprising, since their neighbor was Colony 4, the other medium-aggression build. The only difference between these two was that 4 has high energy, and 5 has high freedom. The major factor that seems to have given 4 the upper-hand is that they had a much heavier flow of ants to the store of 70-quality food placed behind their starting point. This was good news to me, since I placed those long skinny halls back there specifically to (hopefully) benefit high-energy (and possibly low-freedom) ants that could make the journey, and wasn't sure if I had made them long or difficult enough.
It took a while, but Colony 5's numbers dwindled at the same rate that 4's climbed. Colony 4 was the 3rd to reach population cap while 5 was the 3rd to fall very shortly thereafter.
At this point it was mostly 1 who was making a run for the center food store, Followed by colony 7. Both colonies kept trying to enter only from the left side, which led to some pretty intense conflict, seeing as these were the two max-aggressive colonies. Just like the conflict between colony 4 and 5 before them, the only difference between these colonies was that colony 1 had higher freedom, and colony 7 had higher energy.
From what you can see in the pathing made by each colony, it looks as though 1's high freedom caused a group of ants to start swarming down near 7's exit, where they patrolled around in circles like some sort of death squad, picking off the steady stream of 7's ants as they tried to either get to or from the center food store.
Meanwhile, 3 was slowly picking away at 2, slowly building up in the same manner that 4 and 5 did. 3 Had a strong flow to the high-value store down the hall, which 2 had yet to develop in any meaningful way. Although, I believe the upper hand was gained by 3 by means of having medium aggression over 2's low aggression, in addition to one thing threatening them both: They bad both lasted so long to this point that their food stores depleted to nothing, aside from the large store down the long hallway, which meant venturing out to the center food store where Colony 1 and 4 had already developed a decent flow.