The HTTP GET method is idempotent: it should behave the same way on multiple accesses.
A single use link, e.g. for resetting a password or confirming a subscription, will usually show a webpage with a form that does a POST. Once that POST has been performed, the single use link is used up.
Single use links will mostly have a one-time secret that should not be leaked. Mails that contain such links or any sensitive information should be encrypted.