The real issue here is that this is evidence in a homicide trial where you need accurate evidence to prove beyond reasonable doubt that the events really happened the way they're argued by the parties and it's just not possible in some cases.
To try to answer the question, yes it does/could. Although it depends much on the interpolation algorithm used to enlarge the image or if there was another process that could produce significant alteration such as an AI assisted process.
If you use interpolation to "make it clearer" you're adding information to it and it stops being an accurate representation of the original. It might look clearer to you and it could help you gain more insight, but after the enhancement it's no longer an accurate representation of the original. The very meaning of the word interpolation is "the insertion of something of a different nature into something else."
As an example imagine there's a far away video of someone handing a phone to another person and you take the best frame you can for analysis. In the original it looks like that person blur is handing something to the other person but it's just a blackish line.
After processing with different algorithms in one it now looks like the person is pointing a gun, in the other shows an empty hand and the AI one for some reason shows it's a bag, and so on.
More specifically about the trial the expert witness declared that he does not actually know how the algorithms used work and that he didn't compare with the original. Which I personally find baffling because this is a laboratory that analyzes evidence that may decide the future of a lot of people's life. The technician needs to know how it works in order to be able to reach an objective conclusion that what they're presenting is accurate.