Amplifying DNA is useful/necessary for all sorts of biological procedures. High concentrations of DNA are needed for efficiently transfecting microbes, for example.
These days, it's also pretty rare that you won't know the complete genome of the species you're working with, and there are free web tools that can help you design unique primers.
there are free web tools that can help you design unique primers.
True, but if you are going to your own PCR, you might as well do your own primer design. It's practically the "hello, world!" of bioinformatics, not to mention that a primer design server might not be able to help you if you're trying to do something fancy.Tito OpenPCR
In the dry lab, I'm really more of a regular PCR consumer than a regular PCR user, but yeah, my attitude towards PCR is more or less my attitude towards snakes: try to keep a respectful distance :-)
What that means is this: you have a sample of some DNA or protein. Typically it might be only one copy or only a handful of copies. Nothing in bioscience works on tiny copies; you typically need thousands or millions, particularly for operations like dna sequencing. PCR allows you to amplify -- ie copy -- your sample by causing the DNA helix to split from heat, including a polymerase or enzyme that does the replication work, including spare dna bases, then cooling to cause the helixes to reform. It's essentially a doubling operation; you run k heating/splitting/replication/cooling cycles and at the end hopefully have (#{ starting copies }) * 2^k
Doing the previous operation requires a pcr device which very accurately and evenly heats and cools a microtitre plate; the polymerase; something to dump all this into like very clean water; and raw dna base pairs.