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Have they tried introducing these worms into drosophila or even mice? I’d be curious what the effect would be.

Also what effect does it have in the birds that carry it?

On the first question, my guess is that it activates queen like behavior on a default ant. So it's not actually giving it powers beyond its species, just reconfiguring the particular setup.

What if it's a male ant?

According to Wikipedia, in larger colonies most ants are sterile, wingless females:

"Larger colonies consist of various castes of sterile, wingless females, most of which are workers (ergates), as well as soldiers (dinergates) and other specialised groups."

Yes, the parent poster understood that, and was asking about the males specifically because they are relatively rare.

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