Yes of course, T is always measured in the mornings as thats when its peaking. Ive probably measured my T around 5-10 times before getting on TRT. Mind you I am in my 20s, everyone was shocked. Also, not only Total T but measured Prolactin, did a contrast brain MRI, SHBG, E2, Albumin, Free T, Thyroid profile, Calcium, and a bunch of other things probably.