You're mixing up 'premature optimisation' and 'unnecessary optimisation'. The first is making code faster that isn't the bottleneck, or dominating performance factor. The second is making something faster than it needs to be. Profiling helps avoid the first, benchmarking helps avoid the second. Both require working (toy) systems, which is hard when you are evaluating which language to begin work in.

Writing a library in C (for reasons of performance) where performance would have been 'good enough' in Python is unnecessary optimisation. Writing your own GC implementation layer in Python because you think that's the bottleneck would be premature optimisation.

EDIT: typo