That's an interesting question. I'm not sure how you would pump CO2 into a bag and kill a colony. I'm not sure it's an accepted process for doing that. Maybe there's a chance you might accidentally euthanise yourself.

I imagine at the start of the process, he was hoping to just kill the queen, and the entire colony would survive. In that case the other bees would replace the queen, with an emergency queen cell, if this was to happen the queen would probably be as aggressive as the previous generation.

But he mentions that he intends on introducing a queen himself. In which case, I would have thought the colony would calm down.

But I have no idea how much by, because I've never been in this situation.