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I'm not sure if defaulting to 32-bit instructions would still allow calling BIOS, but good point!

In this case the design goal was to show the easiest way to jump into C and start building the actual application, so I just tried to avoid any trickery (even enabling the A20 line)

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You just set your chosen segment register(s) in protect mode, jump back to real mode, and then can use this segment register(s) in combination with the 32bit prefix to make a 32 bit memory access. All other segment registers are untouched and you can call into the bios just fine.

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