With a self powered bus (any technology) running on standard streets you can have it go anywhere. If the route you choose is wrong most of the costs were the bus so you can start a different route in a different area until you get people riding. If there is a popular event one weekend you can shift buses to serve that event for just that weekend. If the needs change you shift routes to match instead of sticking with routes that no longer make sense.
Don't get me wrong, advantages of fixed wires and rails (note the and!) make it worthwhile to put in a trolley system on your busiest routes. However the advantage of a bus that can go anywhere means that in general no you can't/shouldn't.